Stages of Early Embryonic Development in Ciona intestinalis
Stages of Early Embryonic Development in Ciona intestinalis (PDF)
Timelapse Movies of Ciona intestinalis Embryonic Development
18 deg C batch1 |
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(142M) |
18 deg C batch2 |
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(96M) |
18 deg C batch3 |
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(file size: 62M) |
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| 18 deg C chorionated |  |
(76M) |
Download of z-stack Movies (in preparation)
| Time after fertilization at 18 degree centigrade | % hatch | Hotta's Stage | ||
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I. Zygote period (0-0.9hr) | |||
| 0.4hpf | 24min | 3% | St. 1 One cell Zygote, fertilized egg | Download |
| 0.8hpf | 48min | 5% | ||
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II. Cleavage period (0.9-4.5hr) | |||
| 0.9hpf | 55min | 6% | St. 2 2-cell | Download |
| 1.45hpf | 1hr 27min | 8% | St. 3 4-cell | Download |
| 1.7hpf | 1hr 42min | 9.70% | ||
| 1.9hpf | 1hr 54min | 11% | St. 4 8-cell | Download |
| 2.35hpf | 2hr 21min | 13% | St. 5a early 16-cell | Download |
| 2.65hpf | 2hr 39min | 15% | St. 5b late 16-cell | Download |
| 3hpf | 3hr | 17% | St. 6a early 32 cell | Download |
| 3.2hpf | 3hr 12min | 18% | St. 6b late 32 cell | Download |
| 3.35hpf | 3hr 21min | 19% | St. 7 44-cell | Download |
| 4hpf | 4hr | 23% | St. 8 64-cell | Download |
| 4.2hpf | 4hr 12min | 24% | St. 9 76-cell | Download |
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III. Gastrula Period (4.5-6.3hr) | |||
| 4.5hpf | 4hr 33min | 26% | St. 10 110-cell, initial gastrula | Download |
| 4.6hpf | 4hr 36min | 26% | Download | |
| 4.9hpf | 4hr 54min | 28% | St. 11 early gastrula | Download |
| 5.65hpf | 5hr 39min | 32% | St. 12 mid gastrula | Download |
| 5.9hpf | 5hr 55min | 34% | St. 13 late gastrula | Download |
| IV. Neurula Period (6.3-8.5hr) | ||||
| 6.35hpf | 6hr 21min | 36% | St. 14 early neurula | Download |
| 6.8hpf | 6hr 48min | 39% | St. 15 mid neurula | Download |
| 7.4hpf | 7hr 24min | 42% | St. 16 late neurula | Download |
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V. Tailbud Period (8.45-17.5hr) | |||
| 8.45hpf | 8hr 27min | 48% | St. 17 initial tailbud I | Download |
| 8.8hpf | 8hr 50min | 50% | St. 18 initial tailbud II | Download |
| 9.3hpf | 9hr 19min | 53% | St. 19 early tailbud I | Download |
| 9.5hpf | 9hr 30min | 54% | St. 20 early tailbud II | Download |
| 10hpf | 10hr 2min | 57% | St. 21 mid tailbud I | Download |
| 10.9hpf | 10hr 54min | 62% | St. 22 mid tailbud II | Download |
| 11.9hpf | 11hr 54min | 68% | St. 23 late tailbud I | Download |
| 12hpf | 12hr | 69% | Download | |
| 13.5hpf | 13hr 27min | 77% | St. 24 late tailbud II | Download |
| 15.9hpf | 15hr 54min | 91% | St. 25 late tailbud III | Download |
| 16.5hpf | 16hr 30min | 94% | Download | |
| 17hpf | 17hr | 97% | Download | |
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VI. Larva Period (17.5hr-) | |||
| 17.5hpf | 17hr 30min | 100% | St. 26 hatching larva | Download |
Variation of defelopmental stage for embryos incubated at different temperatures (PDF)
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